| Dayton Freight - Hudson | Trucking company | 3011 Enloe St | Mon–Fri 8 AM–5 PM |
| Dealers Choice | Building materials supplier | 700 Norflex Dr | Mon–Fri 7:30 AM–3:30 PM |
| Dedicated Logistics - Hudson, WI | Trucking company | 2720 Enloe St | Mon–Fri 8 AM–5 PM |
| Derrek Tarter, Rise and Grind Real Estate Group | eXp Realty | Real estate agent | 600 Old State Highway 35 | Daily 9 AM–10 PM |
| Dexsa Company | Gift shop | 1501 Livingstone Rd | Fri 8 AM–4 PM |
| Digital Garage | Computer store | 1810 Webster St #2 | Mon 9 AM–3 PM |
| Discover Hudson | Tourist attraction | 219 2nd St | Mon–Fri 8:30 AM–4:30 PM |
| Discover Strength | Personal trainer | 1007 Pearson Dr | Fri 5:30 AM–8 PM |
| Discovery Financial | Financial planner | 2217 Vine St #205 | Fri 9 AM–5 PM |
| Divine Custom Homes | Custom home builder | 2424 Monetary Blvd | Fri 8 AM–4:30 PM |
| Divine Real Estate Group | Real estate agency | 2465 Sharon Ln | Mon–Fri 10 AM–4 PM |
| DocuCopies.com | Commercial printer | 636 Commerce Dr | Fri 8 AM–7 PM |
| Don Timmerman | Real estate agency | 2501 Hanley Rd | Daily 8 AM–6 PM |
| Donahue Roofing Co. | Roofing contractor | 819 Coulee Rd | Mon–Fri 8:30 AM–5 PM |
| Dragon Pearl | Chinese restaurant | 431 2nd St #202 | Mon 11 AM–9:30 PM |
| Droplets of Wellness, LLC | Metaphysical supply store | 226 Locust St #2 | Tue–Wed 11 AM–6 PM |
| Durham Executive Group | Real estate consultant | 220 Muirfield Trail | Fri 8 AM–7 PM |
| Eagle Storage - Hudson WI | Storage facility | 583 Lenertz Rd | Mon–Fri 10 AM–5 PM |
| Eagle View Glass Works | Glass & mirror shop | 634 Commerce Dr | Mon–Fri 8 AM–5 PM |
| Eckberg Lammers, P.C. | Law firm | 430 2nd St | Mon–Fri 8 AM–5 PM |
| eCycling St. Croix Valley | Recycling center | 900 Industrial St | Mon–Sat 9 AM–2 PM |
| Edelman Financial Engines | Financial planner | 1330 Hosford St Ste b | Fri 8:30 AM–5 PM |
| Edina Realty - Hudson Real Estate Agency | Real estate agency | 400 2nd St S Ste 130 | Mon–Fri 8:30 AM–5 PM |
| Edina Realty/Clint Hetchler | Real estate agent | 400S 2nd St S Ste 130 | Daily 8 AM–11 PM |
| EJ's Pizzeria | Pizza restaurant | 1830 Webster St #150 | Mon–Thu 11 AM–9 PM |
| Elevated Tactical Solutions, LLC | Firearms academy | 109 2nd St | Fri 9 AM–5 PM |
| Eliza Rae Photography | Photographer | 1178 56th St | Daily 9 AM–7 PM |
| Ember and Bean Roasting Co | Coffee roastery | 117 Commercial St | Daily 6:30 AM–4 PM |
| Engraved Connections | Engraver | 1352 Carriage Dr Unit A | Fri 8 AM–5 PM |
| Estreen & Ogland Attorneys At Law | General practice attorney | 304 Locust St | Fri 8:30 AM–5 PM |
| Et Cetera | Gift shop | 502 2nd St | Mon–Fri 11 AM–6 PM |
| Faith Community Church | Church | 777 Carmichael Rd | Mon 9 AM–1 PM |
| Farrell's eXtreme Bodyshaping - Hudson | Nutritionist | 1301 Gateway Cir Ste 400 | Mon–Thu 4:30 AM–10:30 AM, 3 PM–6:30 PM |
| Farrill's Sunrise Nursery and Garden Center | Garden center | 599 Co Rd UU | Fri 8 AM–6 PM |
| Ferguson Plumbing Supply | Plumbing supply store | 639 Brakke Dr | Mon–Fri 7 AM–4 PM |
| Ferrellgas | Propane supplier | 597 Schommer Dr | Fri 8 AM–5 PM |