| Dunn County Human Services | Department of social services | 3001 US-12 #160 | Mon–Fri 8 AM–4:30 PM |
| Dunn County Transit | Transportation service | 640 Stokke Pkwy | Mon–Fri 7 AM–5 PM |
| Dunn County Treasurer Office | Tax department | 3001 US-12 Ste 102A | Fri 8 AM–4:30 PM |
| Dunn County Zoning Administration | County government office | 3001 US Hwy 12 East, Ste 240A | Fri 8 AM–4:30 PM |
| Dunn Energy Cooperative | Electric utility company | N5725 600th St | Mon–Thu 7:30 AM–5 PM |
| Dunnville Barrens State Natural Area | Park | 580th St | Daily Open 24 hours |
| Dunnville State Wildlife Area | Wildlife refuge | Menomonie, WI 54751 | Daily Open 24 hours |
| Dye Candy Fabrics | Quilt shop | E4620 County Rd C | Fri 10 AM–5 PM |
| Eclipse Self Storage - Menomonie (Traditional Storage) | Self-storage facility | N4630 WI-25 | Fri 9 AM–5 PM |
| Eclipse Self Storage, LLC - Menomonie (Climate Controlled) | Self-storage facility | 1501 N Broadway St | Mon–Fri 9 AM–5 PM |
| Eiden & Hatfield, LLC | Law firm | 392 Red Cedar St Ste 5 | Mon–Fri 8 AM–5 PM |
| Ellsworth Cooperative Creamery- Menomonie | Cheese manufacturer | 3001 County Rd B | Daily 8 AM–6 PM |
| Elsenpeter Repair | Truck repair shop | E5484 County Rd BB | Mon 8 AM–5 PM |
| Emerald Ridge Weddings & Receptions | Corporate office | E6341 County Rd J | Fri 9 AM–5 PM |
| Envirotek Forestry Consulting, LLC | Forestry service | E5440 770th Ave | Mon–Fri 8 AM–5 PM |
| Estilo Salon | Hair salon | 313 Main St E | Fri 9 AM–5 PM |
| ETS Menomonie | Fitness center | 541 N Broadway St | Mon–Fri 12 PM–8 PM |
| EXA Sports - Menomonie | Sports school | 2920 59th St | Mon–Thu 5:30 PM–7:30 PM |
| Exit 45 Restaurant | Bakery | 2100 County Rd B | Mon–Sat 6 AM–9 PM |
| Explore Menomonie | Visitor center | 1125 N Broadway St STE 3 | Mon–Fri 8 AM–5 PM |
| Express Employment Professionals | Employment agency | 1915 Wilson St | Fri 8 AM–5 PM |
| Extension Dunn County Office | County government office | 3001 US-12 Ste 216 | Mon–Fri 8 AM–4:30 PM |
| Fairchild Law Office | General practice attorney | 508 Wilson Ave | Fri 8:30 AM–4 PM |
| Faze’s Tobacco | Tobacco shop | 214 Main St E | Daily 9 AM–9 PM |
| Fiber Lab | Fabric store | 301 Main St E Suite A | Tue 10 AM–7 PM |
| Fiesta Cantina Authentic Mexican Food | Mexican restaurant | 1705 Plaza Dr | Mon–Fri 10 AM–10 PM |
| First Congregational-UCC | United church of christ | 420 Wilson Ave | Mon–Thu 9 AM–2 PM |
| Fitness 1440 Menomonie | Fitness center | 1501 N Broadway St | Mon–Thu 9 AM–8 PM |
| Five Below | Variety store | 1501 N Broadway St Ste 5 | Mon–Sat 10 AM–9 PM |
| Fleet Feet | Screen printer | 310 Main St E | Tue–Thu 9 AM–6 PM |
| Fleet XL Sales and Leasing | Trailer repair shop | 1415 Indianhead Dr E | Mon–Fri 7 AM–5 PM |
| Floyd of all Trades Handyman Services LLC | Contractor | N7450 537th St | Fri 7 AM–5 PM |
| Foos Wine Club | Wine store | 332 Main St E | Thu–Fri 3 PM–8 PM |
| Ford Motor Co | Auto parts store | 5700 Badger Dr | Mon–Fri 8:30 AM–10 PM |
| Forma | Design agency | 110 Main St W | Fri 9 AM–5 PM |
| Foxwalk Sawmill | Building materials supplier | 570th Ave | Mon–Fri 8 AM–4 PM |